Equipment

 
Lightsheet.Z1 Microscope
 Zeiss Lightsheet  Short-Term Use

Long-term,high-speed 3D imaging with virtually no phototoxicity or bleaching may sound too good to be true, but the Lightsheet.Z1 delivers. Capable of imaging both aqueous (RI=1.33) and cleared tissue (RI=1.45) samples, this microscope This revolutionary microscope aquires 3D images that previously took hours on a point scanner in mere minutes! The ability to freely rotate your sample and image from multiple angles (multi-view), improves resolution and imaging depth.

Laser lines: 405, 488, 561, and 638
Commonly imaged dyes: DAPI. BFP, GFP, 488, tdTomato, dsRed, mCherry, 555, 568, 594, 647
RI=1.33 optics: 5x/0.16, 20x/1.0, 40x/1.0
RI=1.45 optics: 5x/0.16, 20x/1.0 (5.5mm working distance)

Lightsheet consumables list
short-term (Mon&Tues) calendar
time-lapse (Wed-Sun) calendar
Lightsheet processing station calendar

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ELYRA Super-Resolution Microscope

 Zeiss ELYRA

Widefield, laser widefield, TIRF, spectral confocal imaging, and super-resolution? The ELYRA can do it all! Using structured illumination, this system can improve resolution in X, Y, and Z two-fold – with no special sample prep needed. If that isn’t sufficient for your experiment, photomanipulation super-resolution techniques like PALM or dSTORM can improve your resolution by as much as an order of magnitude! Clicking a button in the ZEISS software easily switches between imaging modes, allowing you to image the same sample by a variety of techniques.

Laser lines SIM/PALM/STORM: 405, 488, 561, and 638
Laser lines confocal: 405, 440, 458, 488, 514, 561, 633
Commonly imaged dyes SIM/PALM/STORM: DAPI. BFP, GFP, 488, tdTomato, dsRed, mCherry, 555, 568, 594, 647
Commonly imaged dyes confocal: all visible spectrum and near IR dyes (405-700nm)

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LSM 880 Confocal Microscope

The LSM 880 confocal microscope offers 34-channels of supreme sensitivity, owing to its GaAsP detectors with the added bonus of a super-resolution Airyscanner. This extraordinary point scanner can simultaneously image and spectrally separate up to 10 different dyes, create high resolution 3D images over large tiled areas, or image single molecules through fluorescence correlation techniques. The unparalleled sensitivity of this system also makes it the ideal live-cell imaging point scanner, allowing for high-speed confocal acquisition with brilliant signal to noise ratios. The Airy Scan array detector removes the requirement for a pinhole allowing ALL of the light collected from your sample to reach the detector. And additional deconvolution provides a 1.7x improvement over the theoretical diffraction limit.

Laser lines: 405, 458, 488, 514, 561, 594, 633
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)


weekday reservation calendar (Mon-Fri, 9-5)
off-hours reservation calendar (evenings, nights and weekends)
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LSM 780 NLO Multi-Photon Microscope

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Multi-photon illumination has always appealed to biologists for imaging deep into living tissue. But did you ever imagine probing more than 5mm into your sample? Using the 710 NLO with our specialized SCA/E objective, you can visualize deep into clarified tissue with incredible resolution. Even without clarifying your samples, this microscope excels at penetrating deep into live or fixed specimens, and creating beautiful 3D datasets.

Laser lines (confocal): 405, 440, 458, 488, 514, 561, 633
Laser lines (2-photon): Mai-Tai DeepSee HP tunable - 680-1040nm
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)
2-photon detection: 34 channel spectral detector or 4 channel (Blue, Green, Red, Far-Red) non-descanned detection


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Axio Scan.Z1

 

Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z1 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. Load your slides, let the software automatically locate your tissue sections, and off you go!

Filter sets: triple band pass (Cyan, Yellow, Red), quadruple bandpass (Blue, Green, Red, Far-Red)
Best dye combinations: DAPI/BFP, GFP/488, tdTomato/dsRed/mCherry/555/568/594, 647
or CFP, YFP, tdTomato/dsRed/mCherry/555/568/594/647 


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AxioZoom V16 Zoom Microscope
 AxioZoom V16

Combining a 16x zoom with high numerical aperture objectives, the AxioZoom.V16 offers superior brightness in large object fields. This highly versatile microscope can image samples illuminated by transmitted, reflected, polarized, and fluorescent light sources, and is equipped with a high resolution color camera and a Hamamatsu Flash 4.0 v2 sCMOS monochrome camera for fast, highly sensitive imaging. The AxioZoom.V16 also boasts an Apotome, an optical sectioning device, capable of creating large field 3D images.

Filter sets: DAPI, CFP, GFP, YFP, RFP, Far Red
Best dye combinations: DAPI/BFP, GFP/488, tdTomato/dsRed/mCherry/555/568/594, 647
or CFP, YFP, tdTomato/dsRed/mCherry/555/568/594/647 


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Cell Observer Live Cell Microscope
 Zeiss Cell Observer Short-Term Use

Trying to reproduce physiological conditions for cells in a petri dish - or even a 300+ well plate -  is always a challenge, but the CELL OBSERVER system makes it easy! This inverted microscope is fully enclosed in a heated incubator box, and features a heated stage with a humidified CO2 microenvironment. And if you’re interested in long term imaging, feel free to ask us about Definite Focus, the focus maintenance system which makes sure your sample doesn’t drift over time. In addition to all this, the system has an Apotome unit for performing grid confocal optical sectioning and a Hamamatsu Flash 4.0 v2 sCMOS camera!

Filter sets: DAPI, CFP, GFP, YFP, Cy3/tdTomato, RFP, Far Red, dual band pass GFP/RFP
Best dye combinations: DAPI/BFP, GFP/488, Cy3/tdTomato/dsRed/mCherry/555/568/594, 647
or CFP, YFP, tdTomato/dsRed/mCherry/555/568/594/647


short-term (Mon&Tues) calendar
time-lapse (Wed-Sun) calendar
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LSM 700 Confocal Microscope
 LSM 700 Inverted Confocal

So you have a four-color sample that requires 3D imaging over large tiled areas? Then the LSM 700 is the microscope for you. This incredibly user-friendly point scanner can image four channels simultaneously, due to its unique Variable Secondary Dichroic, all the while acquiring 3D images over multiple fields of view.

Laser lines: 405, 488, 555, 638
Commonly imaged dyes: DAPI/BFP, GFP/488, 555/568/tdTomato, 647


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PALM Laser Microdissection Microscope

 PALM Laser Microdissection Microscope

Even the world’s greatest surgeon might struggle with dissecting single cells out of a slice of tissue. With the PALM system, laser microdissection, catapult and capture are as easy as drawing a region to dissect on the screen, and pressing start. Remove individual cells from your samples slices, or excise entire subregions for RT-PCR, DNA, or even mass spec analysis.

Laser lines: 350
Filtersets: DAPI, GFP, RFP


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LSM 510 Inverted Confocal

 LSM 510 Inverted Confocal


The LSM 510 META offers the flexibility of both spectral and traditional filter based fluorescence 3D imaging on the same system. With a few simple clicks in the ZEISS software, you can toggle between using the wide array of emission filters on the system to imaging on the META spectral detector, allowing you to image up to 8 channels simultaneously. The 510 also offers an independent pinhole for each of its three detectors, giving you greater flexibility over the thickness of your optical sections.

Laser lines: 405, 458, 488, 514, 543, 633
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)


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LSM 510 Upright Confocal
 LSM 510 Upright Confocal

The LSM 510 META offers the flexibility of both spectral and traditional filter based fluorescence 3D imaging on the same system. With a few simple clicks in the ZEISS software, you can toggle between using the wide array of emission filters on the system to imaging on the META spectral detector, allowing you to image up to 8 channels simultaneously. The 510 also offers an independent pinhole for each of its three detectors, giving you greater flexibility over the thickness of your optical sections.

Laser lines: 458, 488, 514, 543, 633
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)


reservation calendar
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Pascal Inverted Confocal Microscope

 Zeiss LSM Pascal Inverted

The Pascal is a very user-friendly two-channel confocal, capable of imaging over large areas and 3D imaging. This system uses traditional filter-based fluorescence emission, and has a separate pinhole for each detector – offering greater control over your optical section thickness.
Laser lines: 405, 458, 488, 514, 543
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)
Spinning Disk Confocal
 Spinning Disk Confocal

Do you need high-resolution confocality? Is it taking too long to scan your thick slides on a point scanner? Is you sample thick and moving fast? Then the HCBI's spinning disk system may be just what you need!

Laser lines: 491, 561
Commonly imaged dyes: GFP, RFP

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Graphics Workstation 1: Image Processing

High-end PC for graphics work running Arivis, Imaris, Volocity, Fiji(ImageJ), and ZEN 2 lite. System can be used for 3D rendering, quantitation and analysis of data.

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Graphics Workstation 2: ZEN

http://www.protel.co.nz/ic/3028504028/hp-z280-angle-700x700.jpg

High-end PC for processing work which includes up-to-date versions of ZEN Black and ZEN Blue with all modules enabled. The station is primarily used for processing 3DSIM and PALM/STORM data and conversion of AxioScan files to .tif/.bigtif format.


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Graphics Workstation 3: Lightsheet

http://www.protel.co.nz/ic/3028504028/hp-z280-angle-700x700.jpg
High-end PC for graphics work running ZEN Black Lightsheet edition and Arivis 4D Vision. The computer features 30TB of data storage and is EXCLUSIVELY used for processing data sets acquired on the Lightsheet microscope.