Lightsheet.7 Microscope
Long-term,high-speed 3D imaging with virtually no phototoxicity or bleaching may sound too good to be true, but the Lightsheet.7 delivers. Capable of imaging both aqueous (RI=1.33) and cleared tissue (RI=1.45) samples, this revolutionary microscope acquires 3D images that previously took hours on a point scanner in mere minutes! The ability to freely rotate your sample and image from multiple angles (multi-view), improves resolution and imaging depth.
Laser lines: 405, 488, 561, and 638
Commonly imaged dyes: DAPI. BFP, GFP, 488, tdTomato, dsRed, mCherry, 555, 568, 594, 647
RI=1.33 optics: 5x/0.16, 20x/1.0, 40x/1.0
RI=1.45 optics: 5x/0.16, 20x/1.0 (5.5mm working distance)
Reservations
Lightsheet consumables list
Vizgen MERSCOPE
ELYRA7 Super-Resolution Microscope
Widefield, laser widefield, TIRF, and super-resolution? The ELYRA can do it all! Using lattice structured illumination, this system can improve resolution in X, Y, and Z two-fold – with no special sample prep needed. The lattice illumination pattern is especially gentle for live-cell imaging. If that isn’t sufficient for your experiment, single molecule localization (SML) super-resolution techniques like PALM or dSTORM can improve your resolution by as much as an order of magnitude.
Laser lines SIM/PALM/STORM: 405, 488, 561, and 638
Commonly imaged dyes SIM/PALM/STORM: DAPI. BFP, GFP, 488, tdTomato, dsRed, 555, 568, 647
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LSM 980 Confocal Microscope with Airyscan2
The LSM 980 confocal microscope offers 34-channels of supreme sensitivity, owing to its GaAsP and Airy scan detectors. This extraordinary point scanner can simultaneously image and spectrally separate up to 10 different dyes, and create high resolution 3D images over large tiled areas. The unparalleled sensitivity of this system also makes it the ideal live-cell imaging point scanner, allowing for high-speed confocal acquisition with brilliant signal to noise ratios. Along with deconvolution Airyscan provides a 1.7x improvement over the theoretical diffraction limit.
Commonly imaged dyes: all visible spectrum (with the exception of cyan), and near IR dyes (405-700nm)
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LSM 880 Confocal Microscope with FLIM
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)
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LSM 980 NLO Multi-Photon Microscope
Multi-photon illumination has always appealed to biologists for imaging deep into living tissue. But did you ever imagine probing more than 5mm into your sample? Using the 980 NLO with our specialized SCA/E or CLARITY objectives, you can visualize deep into clarified tissue with incredible resolution. Even without clarifying your samples, this microscope excels at penetrating deep into live or fixed specimens, and creating beautiful 3D datasets thanks to a tunable infra-red laser that can be used for multi-photon (2P, 3P) and second harmonic generation (SHG) imaging.
Laser lines (confocal): 405, 440, 458, 488, 514, 561, 633
Laser lines (2-photon): Spectra-Physics Insight DS+ tunable from 680-1300nm
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)
Confocal detection: 34 channel spectral detector
2-photon detection: 34 channel spectral detector or 4 channel (Blue, Green, Red, Far-Red) non-descanned detection
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Axio Scan.Z7
Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z7 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. Load your slides, let the software automatically locate your tissue sections, and off you go! Whole slide imaging along with barcode recognition allows simple intergration into a digital pathology workflow.
Best dye combinations: DAPI/488/568/647; CFP/YFP/mCherry
Reservations
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Axio Scan.Z7 #2
Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z1 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. This particular system is configured for maximum color seperation, an additional infra-red imaging channel and the ability to perform polarized light imaging as well.
Best dye combinations: DAPI/488/568/647/750
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AxioZoom V16 Zoom Microscope
Best dye combinations: DAPI/BFP, GFP/488, tdTomato/dsRed/mCherry/555/568/594, 647
or CFP, YFP, tdTomato/dsRed/mCherry/555/568/594/647
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LSM 900 Live Cell Confocal Microscope
Commonly imaged dyes: DAPI, Alexa 488/GFP, Alexa 555/568/tdTomato, Alexa 647
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LSM 700 Confocal Microscope
So you have a four-color sample that requires 3D imaging over large tiled areas? Then the LSM 700 is the microscope for you. This incredibly user-friendly point scanner can image up to two channels simultaneously, due to its unique Variable Secondary Dichroic, all the while acquiring 3D images over multiple fields of view. In addition, the LSM 700 boasts a back-thinned Hamatsu EMCCD camera for performing high-sensitivity wide-filed imaging.
Laser lines: 405, 488, 555, 638
Commonly imaged dyes: DAPI/BFP, GFP/488, 555/568/tdTomato, 647
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LSM 900 Confocal Microscope
Commonly imaged dyes: DAPI, Alexa 488/GFP, Alexa 555/568/tdTomato, Alexa 647
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PALM Laser Microdissection Microscope
Even the world’s greatest surgeon might struggle with dissecting single cells out of a slice of tissue. With the PALM system, laser microdissection, catapult and capture are as easy as drawing a region to dissect on the screen, and pressing start. Remove individual cells from your samples slices, or excise entire subregions for RT-PCR, DNA, or even mass spec analysis.
Laser lines: 350
Filtersets: DAPI, GFP, RFP
X-CLARITY Tissue Clearing System
Are you tired of waiting for your tissues to passively clear? Speed up the Process with the X-CLARITY Tissue Clearing System. Perform the lipid removal step of the CLARITY protocol in as little as3 hours (2mm slice) or 24 hours (Whole brain). ETC buffer is included with your reservation!
Reservations
HCBI CLARITY protocol
X-CLARITY User Guide
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LifeCanvass SmartClear Pro Tissue Clearing Sysytem
Are you tired of waiting for your tissues to passively clear? Speed up the Process with the SmartClear Pro Tissue Clearing System. Perform the lipid removal step of the CLARITY protocol in as little as 3 hours (2mm slice) or 24 hours (Whole brain). ETC buffer is included with your reservation!
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x-claritytm_tissue_clearing_system_user_manual_ver_1.8.2_logos.pdf | 1.39 MB | |
light_sheet_consumables_document.pdf | 73 KB |