Equipment - Biological Labs, 16 Divinity Ave., Cambridge

 

Lightsheet.7 Microscope

 Zeiss Lightsheet7

Long-term,high-speed 3D imaging with virtually no phototoxicity or bleaching may sound too good to be true, but the Lightsheet.7 delivers. Capable of imaging both aqueous (RI=1.33) and cleared tissue (RI=1.45) samples, this revolutionary microscope acquires 3D images that previously took hours on a point scanner in mere minutes! The ability to freely rotate your sample and image from multiple angles (multi-view), improves resolution and imaging depth.

Laser lines: 405, 488, 561, and 638
Commonly imaged dyes: DAPI. BFP, GFP, 488, tdTomato, dsRed, mCherry, 555, 568, 594, 647
RI=1.33 optics: 5x/0.16, 20x/1.0, 40x/1.0
RI=1.45 optics: 5x/0.16, 20x/1.0 (5.5mm working distance)


Reservations
Lightsheet consumables list

top

__________________________________________________________________________

Vizgen MERSCOPE

 VizGen Unveils MERSCOPE

The MERSCOPE combines MERFISH technology with high resolution imaging, fluidics, image processing, and automation to deliver a complete end-to-end spatial genomics solution. The MERSCOPE Platform comes with everything you need to easily aquire and analyze high quality MERFISH measurements.
 
top
 
________________________________________________________________________

ELYRA7 Super-Resolution Microscope

ZEISS Elyra 7 with Lattice SIM with SMLM

Widefield, laser widefield, TIRF, and super-resolution? The ELYRA can do it all! Using lattice structured illumination, this system can improve resolution in X, Y, and Z two-fold – with no special sample prep needed. The lattice illumination pattern is especially gentle for live-cell imaging. If that isn’t sufficient for your experiment, single molecule localization (SML) super-resolution techniques like PALM or dSTORM can improve your resolution by as much as an order of magnitude. 

Laser lines SIM/PALM/STORM: 405, 488, 561, and 638
Commonly imaged dyes SIM/PALM/STORM: DAPI. BFP, GFP, 488, tdTomato, dsRed, 555, 568, 647

Details

top

__________________________________________________________________________

LSM 980 Confocal Microscope with Airyscan2

The LSM 980 confocal microscope offers 34-channels of supreme sensitivity, owing to its GaAsP and Airy scan detectors. This extraordinary point scanner can simultaneously image and spectrally separate up to 10 different dyes, and create high resolution 3D images over large tiled areas. The unparalleled sensitivity of this system also makes it the ideal live-cell imaging point scanner, allowing for high-speed confocal acquisition with brilliant signal to noise ratios. Along with deconvolution Airyscan provides a 1.7x improvement over the theoretical diffraction limit.

Laser lines: 405, 488, 514, 561, 594, 640
Commonly imaged dyes: all visible spectrum (with the exception of cyan), and near IR dyes (405-700nm)

Reservations

top

__________________________________________________________________________

LSM 880 Confocal Microscope with FLIM

A top of the line fully spectral point scanning workhorse, the LSM 880 can handle all of your fixed-sample imaging demands. With a 440nm laser line, this confocal is particularly suited to imaging "cyan" dyes and fluorescent proteins. It is also equiped with pulsed 405 and 440 lasers and a 2-channel Big.2 detector for performing Fluorescence Lifetime Imaging (FLIM) measurements. The system is allso equiped for imaging single molecules through fluorescence correlation techniques.
 
Laser lines: 405, 440, 458, 488, 514, 561, 594, 633
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)

Reservations

top
__________________________________________________________________________

LSM 980 NLO Multi-Photon Microscope

 
http://www.azom.com/images/Article_Images/ImageForArticle_8222(1).jpg

Multi-photon illumination has always appealed to biologists for imaging deep into living tissue. But did you ever imagine probing more than 5mm into your sample? Using the 980 NLO with our specialized SCA/E or CLARITY objectives, you can visualize deep into clarified tissue with incredible resolution. Even without clarifying your samples, this microscope excels at penetrating deep into live or fixed specimens, and creating beautiful 3D datasets thanks to a tunable infra-red laser that can be used for multi-photon (2P, 3P) and second harmonic generation (SHG) imaging.

Laser lines (confocal): 405, 440, 458, 488, 514, 561, 633
Laser lines (2-photon): Spectra-Physics Insight DS+ tunable from 680-1300nm
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)
Confocal detection:
34 channel spectral detector
2-photon detection: 34 channel spectral detector or 4 channel (Blue, Green, Red, Far-Red) non-descanned detection

Reservations

top
__________________________________________________________________________

Axio Scan.Z7

 

Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z7 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. Load your slides, let the software automatically locate your tissue sections, and off you go! Whole slide imaging along with barcode recognition allows simple intergration into a digital pathology workflow.

Cameras: AxioCam 705 Color & Hamamatsu Orca Flash 4.0
Transmitted Light: Brightfield & TIE contrast
Filter sets: quadruple bandpass (Blue, Green, Red, Far-Red), triple bandpass (Cyan, Yellow, Red), DAPI/BFP, 488/GFP, 555/568/tdTomato, 594/mCherry, 647/Cy5
Best dye combinations: DAPI/488/568/647; CFP/YFP/mCherry

Reservations

top

__________________________________________________________________________

Axio Scan.Z7 #2

 

Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z1 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. This particular system is configured for maximum color seperation, an additional infra-red imaging channel and the ability to perform polarized light imaging as well.

Cameras: AxioCam 705 Color & Hamamatsu Orca Flash 4.0
Transmitted Light: Brightfield, TIE contrast, Circular-Polarization
Filter sets: quadruple bandpass (Blue, Green, Red, Far-Red), DAPI/BFP, 488/GFP, 555/568/tdTomato, 594/mCherry, 647/Cy5, 750/Cy7
Best dye combinations: DAPI/488/568/647/750
top

__________________________________________________________________________

AxioZoom V16 Zoom Microscope

 AxioZoom V16
 
Combining a 16x zoom with high numerical aperture objectives, the AxioZoom.V16 offers superior brightness in large object fields. This highly versatile microscope can image samples illuminated by transmitted, reflected, polarized, and fluorescent light sources. It is equipped with a high resolution color camera and a photometrics Prime 95B back-illuminated sCMOS camera for fast, highly sensitive imaging. The AxioZoom.V16 also boasts an Apotome, an optical sectioning device, capable of creating large field of view 3D images.
Cameras: Prime 95B BI, Axiocam 712 color
Filter sets: DAPI, CFP, GFP, YFP, RFP, Far Red
Best dye combinations: DAPI/BFP, GFP/488, tdTomato/dsRed/mCherry/555/568/594, 647
or CFP, YFP, tdTomato/dsRed/mCherry/555/568/594/647 
top
__________________________________________________________________________

LSM 900 Live Cell Confocal Microscope

lsm 900

The LSM 900 is optimal for confocal imaging with the four commonly used dyes. The entire system is fully motorized for autofocusing over large fields of view. It comes with full incubation including CO2 for long term live cell imaging experiments. In addition to the confocal GaAsP detectors, the system has an Axiocam camera and an ESID for widefield fluorescence or transmitted light imaging.
 
Objectives: 5x/0.16 air, 10x/0.45 air, 20x/0.8 air, 40x/1.2 multi-immersion, 40x/1.4 oil
Laser lines: 405, 488, 561, 640
Commonly imaged dyes:
DAPI, Alexa 488/GFP, Alexa 555/568/tdTomato, Alexa 647
 

Reservations

Details

top
__________________________________________________________________________

LSM 700 Confocal Microscope

 LSM 700 Inverted Confocal
 

So you have a four-color sample that requires 3D imaging over large tiled areas? Then the LSM 700 is the microscope for you. This incredibly user-friendly point scanner can image up to two channels simultaneously, due to its unique Variable Secondary Dichroic, all the while acquiring 3D images over multiple fields of view. In addition, the LSM 700 boasts a back-thinned Hamatsu EMCCD camera for performing high-sensitivity wide-filed imaging.

Laser lines: 405, 488, 555, 638
Commonly imaged dyes: DAPI/BFP, GFP/488, 555/568/tdTomato, 647

top
__________________________________________________________________

LSM 900 Confocal Microscope

lsm 900

The LSM 900 is optimal for confocal imaging with the four commonly used dyes. The entire system is fully motorized for autofocusing over large fields of view. It comes with an artificial intelligent (AI) Sample finder that enables the user to map their sample with flexibility to zone in on regions of interest. In addition to the confocal GaAsP detectors, the system has an Axiocam camera and an ESID for widefield fluorescence or transmitted light imaging. 
 
Objectives: 2.5x/0.085, 10x/0.45 air, 20x/0.8 air, 25x/0.8 water, glycerol and oil, 40x/1.2 water, 40x/1.4 oil
Laser lines: 405, 488, 561, 640
Commonly imaged dyes:
DAPI, Alexa 488/GFP, Alexa 555/568/tdTomato, Alexa 647
_________________________________________________________________________

PALM Laser Microdissection Microscope

 PALM Laser Microdissection Microscope
 

Even the world’s greatest surgeon might struggle with dissecting single cells out of a slice of tissue. With the PALM system, laser microdissection, catapult and capture are as easy as drawing a region to dissect on the screen, and pressing start. Remove individual cells from your samples slices, or excise entire subregions for RT-PCR, DNA, or even mass spec analysis.

Laser lines: 350
Filtersets: DAPI, GFP, RFP
top
__________________________________________________

X-CLARITY Tissue Clearing System

 

Are you tired of waiting for your tissues to passively clear?  Speed up the Process with the X-CLARITY Tissue Clearing System.  Perform the lipid removal step of the CLARITY protocol in as little as3 hours (2mm slice) or 24 hours (Whole brain). ETC buffer is included with your reservation!

Reservations
HCBI CLARITY protocol
X-CLARITY User Guide

top

___________________________________________

LifeCanvass SmartClear Pro Tissue Clearing Sysytem

Are you tired of waiting for your tissues to passively clear?  Speed up the Process with the SmartClear Pro Tissue Clearing System.  Perform the lipid removal step of the CLARITY protocol in as little as 3 hours (2mm slice) or 24 hours (Whole brain). ETC buffer is included with your reservation!

Reservations

top

________________________

 

 

 
x-claritytm_tissue_clearing_system_user_manual_ver_1.8.2_logos.pdf1.39 MB
light_sheet_consumables_document.pdf73 KB