This often occurs on the LSM 780, 2-Photon, and ELYRA systems. The acquisition computer has not established communication with the real time computer (RTC).
To avoid this, follow this startup procedure:
1) Turn on "Main Switch"
2) Turn on "Systems/PC"
3) Turn ON computer and wait for it to boot, log in with your RC account
4) Turn ON "Components"
5) Wait 30 seconds, then open ZEN
If ZEN stills stalls at 2% while starting up the system, follow the directions below:
The real time computer is a black computer case on the floor to the right (780, Elyra) or left (2-photon) of the acquisition computer. Although it looks just like a big black computer case, there is a door on the front of the RTC that can be opened. Near the blue light at the top right behind the door, you will see (feel) a reset button. Push this button and wait 30 seconds, ZEN will continue loading (do not click or restart anything!).
Unfortunately, none of the HCBI computer are able to access the internet (including workstations). For HCBI users without HUIT credentials, you can connect to the Harvard Guest Network by following the directions here.
As both of these systems are designed for long-term live cell imaging (potentially up to multiple days), bookings from 9AM Monday - 8PM Tuesday are limited to 4 hours. This ensures access for people performing short-term experiments. From 8PM Tuesday, until 9AM Monday there are no time restrictions on the length of an experiment. Please use the "short-term" calendar for bookings on Mondays and Tuesday, and the "time-lapse" calendar for bookings for the rest of the week.
The HCBI doors are unlocked 9AM-7PM Monday to Friday. If you require access to the facility outside these hours, and have completed your general lab safety training and the commercial laser safety training programs through the EHS website, you can request after-hours access from Sarah Elwell (firstname.lastname@example.org). After-hours access is only granted to undergrads after they have been using the facility for a few weeks and demonstrate that they are well versed in the use of the equipment (this does not apply to courses using the facility).