FAQ

Where are you located?

The HCBI is located in Room 2052 of the Biological Laboratories on Harvard's Cambridge campus.

Click here for a map!

ZEN Black stalls at 2% and will not initialize. How do I fix this?

This often occurs on the LSM 780, 2-Photon, and ELYRA systems.  The acquisition computer has not established communication with the real time computer (RTC). 

To avoid this, follow this startup procedure:

1) Turn on "Main Switch"

2) Turn on "Systems/PC"

3) Turn ON computer and wait for it to boot, log in with your RC account

4) Turn ON "Components"

5) Wait 30 seconds, then open ZEN

If ZEN stills stalls at 2% while starting up the system, follow the directions below:

The real time computer is a black computer case on the floor to the right (780, Elyra) or left (2-photon) of the acquisition computer.  Although it looks just like a big black computer case, there is a door on the front of the RTC that can be opened.  Near the blue light at the top right behind the door, you will see (feel) a reset button.  Push this button and wait 30 seconds, ZEN will continue loading (do not click or restart anything!).

Why are there two calendars for the Cell Observer and Lightsheet microscopes?

As both of these systems are designed for long-term live cell imaging (potentially up to multiple days), bookings from 9AM Monday - 8PM Tuesday are limited to 4 hours.  This ensures access for people performing short-term experiments.  From 8PM Tuesday, until 9AM Monday there are no time restrictions on the length of an experiment.  Please use the "short-term" calendar for bookings on Mondays and Tuesday, and the "time-lapse" calendar for bookings for the rest of the week.

SPINAL is requesting an expense/billing code, how do I get one?

Instructions for requesting a billing code 

 

1. Log into SPINAL (https://webapps.sciences.fas.harvard.edu/spinal/).

2. Under "Resources" locate the microscope you'd like to reserve.

3. Click on the "(NOTE: expense code needed)" link.

4. Complete brief online form. (If you are an undergrad or G1 choose Sarah Elwell as your lab admin!)

5. An email will be sent to the lab admin requesting that they enter the expense code.

6. Once the coding has been entered you will get an email letting you know that you can now make reservations.

7. Every night a script is run that adds your expense code(s) to all microscopes in the HCBI. This eliminates the need to submit coding requests for each microscope you wish to use.

How do I get after-hours access to the HCBI?

The HCBI doors are unlocked 9AM-7PM Monday to Friday.  If you require access to the facility outside these hours, and have completed your general lab safety training and the commercial laser safety training programs through the EHS website, you can request after-hours access from Sarah Elwell (sarah_elwell@harvard.edu)