Should I acknowledge the HCBI in publications?

YES!  This is how we justify our existence and continue to provide you with the best imaging resources available!  We suggest the following text in the acknowledgements section of your paper:

"We thank the Harvard Center for Biological Imaging (RRID:SCR_018673) for infrastructure and support."

If you think a particular staff member(s) went above and beyond, please also mention them by name or consider making them a co-author according to this policy.