Tips and Tricks

Coverslips:


All HCBI coverslip corrected objectives are corrected for #1.5 Coverslips. Please ensure you are ONLY using #1.5 slips.

Mounting Media:

The HCBI recomends Prolong Glass (refractive index = 1.51) for any imaging performed with oil immersion objectives.  For glycerol objectives, or any experiment that requires a non-curing mountant, a number of glycerol-based media are availible.  The higher the refractive index, the better.

For tissue clearing experiments you MUST use the final clearing solution as your mountant!