#  2 Photon Specifications 

 



##  **LSM 980 NLO Multi-Photon Microscope**

 Multi-photon illumination has always appealed to biologists for imaging deep into living tissue. But did you ever imagine probing more than 5mm into your sample? Using the 980 NLO with our specialized SCA/E or CLARITY objectives, you can visualize deep into cleared tissue with incredible resolution. Even without clearing your samples, this microscope excels at penetrating deep into live or fixed specimens, and creating beautiful 3D datasets thanks to a tunable infra-red laser that can be used for multi-photon (2P, 3P) and second harmonic generation (SHG) imaging. A good choice for thick tissue sections, organoids, and organisms such as zebrafish.

###  General Hardware

 **Stand:** Zeiss Axio Examiner Z.1 (upright) **Laser lines (confocal):** 405, 440, 458, 488, 514, 561, 633  
**Laser lines (2-photon):** Spectra-Physics Insight DS+ 3W tunable from 680-1300nm  
**Commonly imaged dyes:** all visible spectrum and near IR dyes (405-700nm)  
**Confocal detection:** 2 PMT + 4 channel GaAsP dectors, T-PMT  
**2-photon detection:** confocal dectors or 4 channel (Blue, Green, Red, Far-Red) non-descanned detection
###  Objectives

 **10x air** (EC Plan-Neofluar 10x/0.30 WD=5.2mm) **25x multi-immersion** (LD LCI Plan-Apochromat 25x/0.8 Imm Corr DIC WD=0.57mm) **10x dipping** (W Plan-Apochromat 10x/0.5 WD=3.7mm (UV) VIS-IR) **20x dipping** (W Plan-Apochromat 20x/1.0 DIC WD=2.3mm, VIS-IR) **20x clearing** (Clr Plan-Neofluar 20x/1.0 Corr nd=1.45 WD=5.6mm)