#  ELYRA7 Super-Resolution Microscope 

 



 Widefield, laser widefield, TIRF, and super-resolution? The [ELYRA](https://www.zeiss.com/microscopy/us/l/campaigns/discover-elyra-7.html?utm_source=google&utm_medium=cpc&utm_campaign=C-00006460&gclid=Cj0KCQjwj5mpBhDJARIsAOVjBdp1WgTBYpOXF3zaAUE7QUtJzxDcg9KJenjC5ahrgM3SZOzaDNYxuLgaAqScEALw_wcB) can do it all! Using lattice structured illumination, this system can improve resolution in X, Y, and Z two-fold – with no special sample prep needed. SIM2 module can provide resolution up to 60nm for the ideal sample. The lattice illumination pattern is especially gentle for live-cell imaging. The system has full incubation (CO2, heating, humidity) and Definite Focus to keep your sample alive and in focus.

 If that isn’t sufficient for your experiment, single molecule localization (SML) super-resolution techniques like PALM or dSTORM can improve your resolution by as much as an order of magnitude.

 **Laser lines SIM/PALM/STORM:** 405, 488, 561, and 638  
**Commonly imaged dyes SIM/PALM/STORM:** DAPI, GFP, mNeonGreen, 488, tdTomato, dsRed, 555, 568, mScarlet, 647

 **Objectives** (as of March 2023):

1. **10x air** (EC Plan-Neofluar 10x/0.30 WD=5.2mm)
2. **25x multi-immersion** (LD LCI Plan-Apochromat 25x/0.8 Imm Corr DIC WD=0.57mm)
3. **40x water** ((LD)C-Apochromat 40x/1.1 W Corr UV-VIS-IR WD=0.62mm)
4. **40x oil** (Plan-Apochroma 40x/1.4 Oil DIC (UV) VIS-IR WD=0.13mm)
5. **63x oil** (Plan-Apochromat 63x/1.40 Oil DIC WD=WD=0.19mm)
6. TIRF 63x oil by request only

 **Filters:**

 Emission:

- Quad 405/488/561/642
- BP 420-480 + BP 495-525 + LP655
- BP 495-550 + BP 570-620
- BP 420-480 + LP655

 Camera Splitters:

- LP560
- BP490-560 + LP640

 **Cameras:** Dual [PCO.edge 4.2 CLHS](https://www.excelitas.com/product/pcoedge-42-clhs-scmos-camera)

   ![Image of specs, can also be found via the link to company webstite](/sites/g/files/omnuum7436/files/styles/hwp_1_1__360x360_scale/public/hcbidoug/files/pco_specs.png?itok=RJt0jwAL) 

 

 **Useful links**

 Youtube video on our SIM function: [https://www.youtube.com/watch?v=gE4-Zl1mTxI&t=2142s](https://www.youtube.com/watch?v=gE4-Zl1mTxI&t=2142s)

 Zeiss database: <https://www.zeiss.com/microscopy/us/local/zen-knowledge-base-home/zen-knowledge-base-elyra.html>

 Sample prep (1st couple pages) [https://hcbi.fas.harvard.edu/files/hcbidoug/files/en\_41\_011\_065\_elyra\_sample-prep-quickguide.pdf](/file_url/122)