#  Nikon Spinning Disk Specifications 

 



## Nikon Spinning Disk CSU-W1


### System Description:

The Nikon Spinning Disk Confocal (CSU-W1) is a fully-loaded live cell imaging platform with dual disks (25 and 50 um) for low and high magnification imaging, dual Photometrics BSI cameras, full incubation, and a programable 405 nm photoconversion/optogenetics laser. This system has full incbuation is ready to image your live samples at top speed.

### Specifications:

- Nikon Ti2E Inverted Microscope
- Yokogawa CSU-W1 dual-disk spinning disk with 25 μm & 50 μm pinholes
- Dual camera
- Motorized stage + piezo drive
- Perfect Focus System
- Full incubation with OKO Labs chamber

### Objectives:

- Plan-Apochromat 4x/0.2 WD=20mm
- Plan-Apochromat 10x/0.45 WD=4mm
- Plan-Apochromat 20x/0.8 WD=0.8mm
- Plan-Apochromat 25x/1.05 Silicon WD=0.55
- Plan-Apochromat 60x/1.42 Oil WD=0.15mm

### Lasers:

Laser Lines: 405,488,561,594,640  
Photomanipulation: 405, 473

### Filters:

Emission Filters:

Sort**Filter**

**Common Fluorophores**

455/50 nm

DAPI/ Alexa 405

525/36 nm

GFP/ Alexa 488

605/52 nm

TRITC/ Alexa 555/ Alexa 568

641/75 nm

mCherry/ Alexa 594

705/72 nm

Cy5/ Alexa 647

Quad

DAPI/FITC/TRITC/CY5





Transmitting Dichroic:

- 405/488/568/647
- 405/488/594

Camera Splitter:

- LP 561

### Cameras:

2 Photometrics [Prime BSI](https://www.photometrics.com/wp-content/uploads/2019/11/BSI-Handbook-E-book-11042019.pdf) sCMOS Cameras 2048x2048, 6.5um pixels

   ![Camera Specs from Photometrics website](/sites/g/files/omnuum7436/files/styles/hwp_1_1__720x720_scale/public/hcbidoug/files/prime_bsi_specs.png?itok=b-YHQPR8)