#  Nikon SoRa Spinning Disk Microscope 

 



### Overview:

The Nikon Super Resolution Spinning Disk Confocal (SoRa) is a fully-loaded live cell imaging platform with dual disks (SoRa 50 μm) for super resolution and standard confocal imaging, Hamamatsu Quest 2 qCMOS & Andor iXon 888 EMCCD and, full incubation. The system is capable of up to 120nm resolution with the SoRa disk and 10s of nm with SMLM. DNA-paint and some dyes are possible for SMLM, but please speak to the imaging team prior to your experiment.

### Specifications:

- Nikon Ti2E Inverted Microscope
- Yokogawa CSU-W1 dual-disk spinning disk with 25 μm & 50 μm pinholes
- Dual camera
- Motorized stage + piezo drive
- Perfect Focus System
- Full incubation with OKO Labs chamber

### Objectives:

- Plan-Apochromat 4x/0.2 WD=20mm
- Plan-Apochromat 10x/0.45 WD=4mm
- Plan-Apochromat 25x/1.05 Silicon WD=0.55mm
- Apochromat Lambda S LWD 40x/1.15 Water WD=0.59mm
- Plan-Apochromat 60x/1.42 Oil WD=0.15mm
- Apochromat TIRF 60x/1.49 Oil WD=0.12

### Lasers:

Laser Lines: 405,488,561,640

### Filters:

Emission Filters:

Sort**Filter**

**Common Fluorophores**

447/60 nm

DAPI/ Alexa 405

520/35 nm

GFP/ Alexa 488

605/52 nm

TRITC/ Alexa 555/ Alexa 568

705/72 nm

Cy5/ Alexa 647

Quad

DAPI/FITC/TRITC/CY5





Transmitting Dichroic:

- 405/488/568/647
- 405/488/568

### Cameras:

Hamamatsu [ORCA-Quest2](https://www.hamamatsu.com/jp/en/product/cameras/qcmos-cameras/C15550-22UP.html) 4096 x 2304 pixels, 4.6 µm pixel size

   ![QE of the Quest 2, with a peak QE of 85% at 450nm](/sites/g/files/omnuum7436/files/styles/hwp_1_1__720x720_scale/public/2025-08/questQE.jpeg?itok=wooHIiPA) 

 

   ![Readout noise profiles of Orca Quest2 compared to Gen2 & 3 sCMOS cameras](/sites/g/files/omnuum7436/files/styles/hwp_1_1__720x720_scale/public/2025-08/quest%20Read%20noise.jpeg?itok=vareHUXC) 

 

[Andor iXon Ultra 888 EMCCD](https://andor.oxinst.com/products/ixon-emccd-camera-series/ixon-ultra-888), 1024x1024pixels, 13um pixel

   ![Spectral profile of QE of iXon comparing to common fluorophores](/sites/g/files/omnuum7436/files/styles/hwp_1_1__720x720_scale/public/2025-08/Screenshot%202025-08-07%20112824.png?itok=G1JiHVQN)