#  Optical Imaging Core @ the Science and Engineering Complex 

 



 ### Welcome to the Optical Imaging Core at the SEC

The Optical Imaging Core is a satilite facility of the Harvard Center for Biological Imaging and provides light microscopy services to researchers in the Allston area.

**Hours:**

The facility is open 24/7 with card access.

A member of the HCBI training team is on site Monday & Wednesday 9am-5pm

**Contact:**  
Optical Imaging Core  
150 Western Ave.  
Room 2.124 SEC  
Boston, MA, 02134  
[hcbi\_imaging@fas.harvard.edu](mailto:hcbi_imaging@fas.harvard.edu)

**Parking Options**

1\) Street Parking along Western Ave

2\) [Prepaid](https://hopps.vpcs.harvard.edu/) Harvard parking lot at 114 Western Ave



 

   ![Optical Imaging Core](/sites/g/files/omnuum7436/files/styles/hwp_1_1__360x360_scale/public/hcbidoug/files/oic_logo.png?itok=Tk0iapuV) 

 

 

 

 ### LSM 900 Bio

This LSM 900 comes fully loaded for all of your bioengineering needs. A Hamamatsu Flash 4.0 camera allows for fast, sensitive widefield fluorescence imaging. The confocal scan head can perform standard multicolor confocal imaging, spectral imaging and create patternned excitation for FRAP/optogenetics experiments. Additionally, an Airyscan 2.0 detector is availible to improve resolution beyond the difration barrier and increase confocal sensitivity. The entire system is fully motorized allowing for autofocusing over large fields of view. Incubation and environmental control will keep your samples happy for the duration of the experiment.

**Objectives: 2.5x/0.085, 10x/0.45 air, 20x/0.8 air, 40x/1.2 water, 40x/1.3 oil**  
**Laser lines: 405, 488, 561, 640**  
**Commonly imaged dyes: DAPI, Alexa 488/GFP, Alexa 555/568/tdTomato, Alexa 647**

**LSM 900 Materials**

This LSM 900 is configured to be a materials workhorse. It comes equiped with black and white and color cameras that can image a wide range of widefield contrasts: DIC, phase contrast, polarization, reflected light and fluorecence. The confocal scan head can image in fluorescence or relected light mode.

**Objectives: 2.5x/0.085 air, 5x/0.3 air, 10x/0.25 air, 20x/0.5 water dipping ,50x/0.55 air,**  
**Laser lines: 405, 488, 561, 640**  
**Commonly imaged dyes: DAPI, Alexa 488/GFP, Alexa 555/568/tdTomato, Alexa 647**

**Axio Scan**

Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z1 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. This particular system is configured with the ability to perform polarized light imaging as well.

**LED excitation: 385, 430, 475, 511, 555, 590, 630 nm**  
**Objectives: 5x/0.16 air, 10x/0.45 air, 20x/0.8 air**  
**Filter sets: quadruple bandpass (Blue, Green, Red, Far-Red), DAPI/BFP, 488/GFP, 555/568/tdTomato, 594/mCherry, 647/Cy5**  
**Best dye combinations: DAPI/488/568/647**



 

   ![LSM 900 Bio](/sites/g/files/omnuum7436/files/styles/hwp_16_9__480x270/public/hcbidoug/files/incubation-stage.jpg?itok=dCknc6Ek)