Live Cell Imaging
Welcome to the Live Cell Imaging Facility
The Live Cell Imaging facility is a satilite facility of the Harvard Center for Biological Imaging and provides light microscopy services to researchers in the Cambridge area.
Hours:
The facility is open 24/7 with card access.
A member of the HCBI training team can be reserved for assistance Monday-Friday 8am-5pm
Contact:
7 Divinity Ave
Sherman Fairchild RM 133
Cambridge, MA 02138
Bruker TruLive 3D Lightsheet
The Bruker TruLive 3D system is designed for long-term,high-speed 3D imaging of live or fixed tissue. This system only allows for imaging of samples in aqueous based media so it is unable to accomodate any cleared tissue sampels. The system utilizes a unique trough samples holder design, allowing or visualization of samples without the need to embed them in agar or other restraining media.
Laser Lines: 405,488,561,642
Objective magnifications 12.5x, 25x, 37.5x, 50x
Nikon CSU-W1 Spinning Disk
The Nikon Spinning Disk Confocal (CSU-W1) is a fully-loaded live cell imaging platform with dual disks (25 and 50 um) for low and high magnification imaging, dual Photometrics BSI cameras, full incubation, and a programable 405 nm photoconversion/optogenetics laser. This system has full incbuation is ready to image your live samples at top speed.
Laser Lines: 405,488,561,594,640
Photomanipulation: 405, 473
Objective magnifications 4x/0.2, 10x/0.4, 20x/0.8, 25x/1.05, 63x/1.42
