The Harvard Center for Biological Imaging was opened May 17, 2010 and is one of the most innovative biological imaging centers in the world. In order to ensure investigators always have access to the latest and best technologies, the HCBI was designed to be an “Evergreen” microscopy facility. Imaging systems at the HCBI are leased rather than purchased and replaced every 2-3 years. This ensures that the facility remains relevant and is never in possession of outdated equipment. Currently, the HCBI contains a variety of microscopes including confocal, super-resolution, multi-photon, lightsheet, live cell imaging, high-content screening, slide scanning, laser dissection and macroscopy. The facility also features wet lab space, eukaryotic and prokaryotic incubation, and access to nearby animal care facilities for a wide range of species, and extensive IT infrastructure and software for image analysis.