Tips and Tricks

Coverslips:


All HCBI coverslip corrected objectives are corrected for #1.5 Coverslips. Please ensure you are ONLY using #1.5 slips.

Mounting Media:

The HCBI recomends Prolong Glass (refractive index = 1.51) for any imaging performed with oil immersion objectives.  For glycerol objectives, or any experiment that requires a non-curing mountant, a number of glycerol-based media are availible.  The higher the refractive index, the better.

For tissue clearing experiments you MUST use the final clearing solution as your mountant!

See also this great document from UCSF (produced bfore Prolong Glass was developed).

hcbi_recommended_mounting_media.pdf256 KB