Equipment (Optical Imaging Core)

LSM 900 Bio

 CLSM - LSM900

This LSM 900 comes fully loaded for all of your bioengineering needs. A Hamamatsu Flash 4.0 camera allows for fast, sensitive widefield fluorescence imaging. The confocal scan head can perform standard multicolor confocal imaging, spectral imaging and create patternned excitation for FRAP/optogenetics experiments. Additionally, an Airyscan 2.0 detector is availible to improve resolution beyond the difration barrier and increase confocal sensitivity. The entire system is fully motorized allowing for autofocusing over large fields of view. Incubation and environmental control will keep your samples happy for the duration of the experiment.

Objectives: 2.5x/0.085, 10x/0.45 air, 20x/0.8 air, 40x/1.2 water, 40x/1.3 oil
Laser lines: 405, 488, 561, 640
Commonly imaged dyes: DAPI, Alexa 488/GFP, Alexa 555/568/tdTomato, Alexa 647

Reservations

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LSM 900 Materials (Arrives March 1, 2021)

Confocal Microscope ZEISS LSM 900 for Materials

This LSM 900 is configured to be a materials workhorse. It comes equiped with black and white and color cameras that can image a wide range of widefield contrasts: DIC, phase contrast, polarization, reflected light and fluorecence. The confocal scan head can image in fluorescence or relected light mode.

Objectives: 2.5x/0.085 air, 5x/0.3 air, 10x/0.25 air, 20x/0.4 air (phase ring) 20x/0.5 air, 40x/1.0 water dipping, 50x/0.55 air,
Laser lines: 405, 488, 561, 640
Commonly imaged dyes: DAPI, Alexa 488/GFP, Alexa 555/568/tdTomato, Alexa 647

Reservations

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Axio Scan

Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z1 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. This particular system is configured with the ability to perform polarized light imaging as well.

LED excitation: 385, 430, 475, 511, 555, 590, 630 nm
Objectives: 5x/0.16 air, 10x/0.45 air, 20x/0.8 air
Filter sets: quadruple bandpass (Blue, Green, Red, Far-Red), DAPI/BFP, 488/GFP, 555/568/tdTomato, 594/mCherry, 647/Cy5
Best dye combinations: DAPI/488/568/647
 

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Graphics Workstation 1: Deconvolution / Intellisis ML Segmentation / Airyscan


High-end storage and analysis PC. Performs standard ZEN Blue functions (stitching, image export), Airyscan processing, deconvolution and Intellisis machine learning segmentation. 

Reservations

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Graphics Workstation 2: ZEN Blue

 

High-end storage and analysis PC. Performs standard ZEN Blue functions (stitching, image export).

Reservations