Equipment

 
Lightsheet.Z1 Microscope
 Zeiss Lightsheet  Short-Term Use

Long-term,high-speed 3D imaging with virtually no phototoxicity or bleaching may sound too good to be true, but the Lightsheet.Z1 delivers. Capable of imaging both aqueous (RI=1.33) and cleared tissue (RI=1.45) samples, this revolutionary microscope acquires 3D images that previously took hours on a point scanner in mere minutes! The ability to freely rotate your sample and image from multiple angles (multi-view), improves resolution and imaging depth.

Laser lines: 405, 488, 561, and 638
Commonly imaged dyes: DAPI. BFP, GFP, 488, tdTomato, dsRed, mCherry, 555, 568, 594, 647
RI=1.33 optics: 5x/0.16, 20x/1.0, 40x/1.0
RI=1.45 optics: 5x/0.16, 20x/1.0 (5.5mm working distance)


short-term (Mon&Tues) calendar
time-lapse (Wed-Sun) calendar
Lightsheet processing station calendar

Lightsheet consumables list

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X-CLARITY Tissue Clearing System

 

Are you tired of waiting for your tissues to passively clear?  Speed up the Process with the X-CLARITY Tissue Clearing System.  Perform the lipid removal step of the CLARITY protocol in as little as3 hours (2mm slice) or 24 hours (Whole brain). ETC buffer is included wit your reservation!

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HCBI CLARITY protocol

X-CLARITY User Guide

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ELYRA Super-Resolution Microscope
 Zeiss ELYRA
 

Widefield, laser widefield, TIRF, spectral confocal imaging, and super-resolution? The ELYRA can do it all! Using structured illumination, this system can improve resolution in X, Y, and Z two-fold – with no special sample prep needed. If that isn’t sufficient for your experiment, single molecule localization (SML) super-resolution techniques like PALM or dSTORM can improve your resolution by as much as an order of magnitude. Clicking a button in the software easily switches between imaging modes, allowing you to image the same sample by a variety of techniques.

Laser lines SIM/PALM/STORM: 405, 488, 561, and 638
Laser lines confocal: 405, 440, 458, 488, 514, 561, 633
Commonly imaged dyes SIM/PALM/STORM: DAPI. BFP, GFP, 488, tdTomato, dsRed, mCherry, 555, 568, 594, 647
Commonly imaged dyes confocal: all visible spectrum and near IR dyes (405-700nm)

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LSM 880 Confocal Microscope with Airyscan

The LSM 880 confocal microscope offers 34-channels of supreme sensitivity, owing to its GaAsP and Airy scan detectors. This extraordinary point scanner can simultaneously image and spectrally separate up to 10 different dyes, create high resolution 3D images over large tiled areas, or image single molecules through fluorescence correlation techniques. The unparalleled sensitivity of this system also makes it the ideal live-cell imaging point scanner, allowing for high-speed confocal acquisition with brilliant signal to noise ratios. Along with  deconvolution Airyscan provides a 1.7x improvement over the theoretical diffraction limit.

Laser lines: 405, 458, 488, 514, 561, 594, 633
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)


weekday reservation calendar (Mon-Fri, 9-5)
off-hours reservation calendar (evenings, nights and weekends)

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LSM 880 Confocal Microscope with FLIM

A top of the line fully spectral point scanning workhorse, the LSM 880 can handle all of your fixed-sample imaging demands. With a 440nm laser line, this confocal is particularly suited to imaging "cyan" dyes and fluorescent proteins. It is also equiped with pulsed 405 and 440 lasers and a 2-channel Big.2 detector for performing Fluorescence Lifetime Imaging (FLIM) measurements.

Laser lines: 405, 440, 458, 488, 514, 561, 594, 633
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)


weekday reservation calendar (Mon-Fri, 9-5)
off-hours reservation calendar (evenings, nights and weekends)

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LSM 880 Upright Confocal
http://www.azom.com/images/Article_Images/ImageForArticle_8222(1).jpg
 

A top of the line fully spectral point scanning workhorse, the LSM 880 can handle your thickest samples with its upright design and long working distance objectives. Full automation allows for the scanning of hundreds of samples. Simply set it and forget it!

Laser lines: 405, 458, 488, 514, 561, 633
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)


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LSM 880 NLO Multi-Photon Microscope
 
http://www.azom.com/images/Article_Images/ImageForArticle_8222(1).jpg

Multi-photon illumination has always appealed to biologists for imaging deep into living tissue. But did you ever imagine probing more than 5mm into your sample? Using the 880 NLO with our specialized SCA/E or CLARITY objectives, you can visualize deep into clarified tissue with incredible resolution. Even without clarifying your samples, this microscope excels at penetrating deep into live or fixed specimens, and creating beautiful 3D datasets thanks to a tunable infra-red laser that can be used for multi-photon (2P, 3P) and second harmonic generation (SHG) imaging.

Laser lines (confocal): 405, 440, 458, 488, 514, 561, 633
Laser lines (2-photon): Spectra-Physics Insight DS+ tunable from 680-1300nm
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)
Confocal detection:
34 channel spectral detector
2-photon detection: 34 channel spectral detector or 4 channel (Blue, Green, Red, Far-Red) non-descanned detection


short-term (Mon&Tues 9AM-7PM) calendar
time-lapse (Wed-Sun) calendar

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Axio Scan.Z1

 

Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z1 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. Load your slides, let the software automatically locate your tissue sections, and off you go!

Filter sets: triple band pass (Cyan, Yellow, Red), quadruple bandpass (Blue, Green, Red, Far-Red)
Best dye combinations: DAPI/BFP, GFP/488, tdTomato/dsRed/mCherry/555/568/594, 647
or CFP, YFP, tdTomato/dsRed/mCherry/555/568/594/647 


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AxioZoom V16 Zoom Microscope
 AxioZoom V16
 

Combining a 16x zoom with high numerical aperture objectives, the AxioZoom.V16 offers superior brightness in large object fields. This highly versatile microscope can image samples illuminated by transmitted, reflected, polarized, and fluorescent light sources. It is equipped with a high resolution color camera and a photometrics Prime 95B back-illuminated sCMOS camera for fast, highly sensitive imaging. The AxioZoom.V16 also boasts an Apotome, an optical sectioning device, capable of creating large field of view 3D images.

Filter sets: DAPI, CFP, GFP, YFP, RFP, Far Red
Best dye combinations: DAPI/BFP, GFP/488, tdTomato/dsRed/mCherry/555/568/594, 647
or CFP, YFP, tdTomato/dsRed/mCherry/555/568/594/647 


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Celldiscoverer7 Live/High Content Microscope
Cell Discoverer
 

Trying to reproduce physiological conditions for cells in a petri dish - or even a 300+ well plate -  is always a challenge, but the CELL DISCOVERER system makes it easy! This fully automated microscope features a heated, humidified, and CO2 regulated microenvironment. And if you’re interested in long term live cell imaging, or high content screening of multiwell plates, this system is for you! The automatic plate loader can hold up to 14 multiwell plates and automatically load/unload them. Rapid deconvolution of images can also be preforemed using the on board NVIDIA M6000 GPU.

Excitation LEDs: 385, 420, 470, 520, 567, 590, 625, 725
Filter sets: triple band pass (Cyan, Yellow, Red), quadruple bandpass (Blue, Green, Red, Far-Red)
Best dye combinations: DAPI/BFP, GFP/488, tdTomato/dsRed/mCherry/555/568/594, 647
or CFP, YFP, tdTomato/dsRed/mCherry/555/568/594/647
 

 

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LSM 700 Confocal Microscope
 LSM 700 Inverted Confocal
 

So you have a four-color sample that requires 3D imaging over large tiled areas? Then the LSM 700 is the microscope for you. This incredibly user-friendly point scanner can image up to two channels simultaneously, due to its unique Variable Secondary Dichroic, all the while acquiring 3D images over multiple fields of view. In addition, the LSM 700 boasts a back-thinned Hamatsu EMCCD camera for performing high-sensitivity wide-filed imaging.

Laser lines: 405, 488, 555, 638
Commonly imaged dyes: DAPI/BFP, GFP/488, 555/568/tdTomato, 647


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PALM Laser Microdissection Microscope
 PALM Laser Microdissection Microscope
 

Even the world’s greatest surgeon might struggle with dissecting single cells out of a slice of tissue. With the PALM system, laser microdissection, catapult and capture are as easy as drawing a region to dissect on the screen, and pressing start. Remove individual cells from your samples slices, or excise entire subregions for RT-PCR, DNA, or even mass spec analysis.

Laser lines: 350
Filtersets: DAPI, GFP, RFP

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Axio Scan.Z1 #2 (Arriving Fall 2018)

 

Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z1 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric or fluorescent dyes. Additional to AxioScan #1, this system can image up to 5 fluorescence channels and polarized light (e.g. congo red staning of collagen)!

Filter sets: Quadruple bandpass (Blue, Green, Red, Far-Red), Single Bandpass: Blue (DAPI/BV421), Green (Alexa 488/GFP), Orange (tdTomato/Cy3/Alexa 555/Alexa 568), Red (Alexa 594, mCherry), Far-red (Cy5, Alexa 647), near-IR (Cy7, Alexa 750) 
Best dye combinations: Nearly anything except CFP, YFP, or spectrally overlapping combinations

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Pascal Inverted Confocal Microscope
 Zeiss LSM Pascal Inverted
 
The Pascal is a very user-friendly two-channel confocal, capable of imaging over large areas and 3D imaging. This system uses traditional filter-based fluorescence emission, and has a separate pinhole for each detector – offering greater control over your optical section thickness.
 
Laser lines: 405, 458, 488, 514, 543
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)

Graphics Workstation 1: Imaris, Volocity

High-end PC for graphics work running Imaris, Volocity, FIJI (ImageJ), and ZEN lite. System can be used for 3D rendering, quantitation and analysis of data. This System is primarily used for image processing/analysis with Imaris and GPU accelerated multiview doconvolution of Lightsheet data using Fiji.
 
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Graphics Workstation 2: ZEN, Cell Profiler

workstation 2

High-end PC for processing work which includes up-to-date versions of ZEN Black and ZEN Blue with all modules enabled, Cell Profiler and FIJI (ImageJ). The station is primarily used for processing Airy Scan, 3DSIM and PALM/STORM data, analysis with Cell Profiler and conversion of AxioScan files to .tif/.bigtif format.


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Graphics Workstation 3: ZEN Black (lightsheet), arivis Vision4D 


High-end PC for graphics work running ZEN Black Lightsheet edition, arivis Vision4D and FIJI (ImageJ). The computer features 30TB of data storage and is primarily used for processing data sets acquired on the Lightsheet microscope.





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Graphics Workstation 4: ZEN, arivis Vision4D, Cell Profiler


High-end PC for graphics work running all madules of ZEN Blue/Black, arivis Vision4D, Cell Profiler and FIJI (ImageJ). The computer features 30TB of data storage and is primarily used for, realtime airyscan processing and GPU accelerated deconvolution in ZEN.




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x-claritytm_tissue_clearing_system_user_manual_ver_1.8.2_logos.pdf1.39 MB
light_sheet_consumables_document.pdf73 KB