HCBI September 2025 Newsletter

News

Zeiss LSM 910 with Lightfield

This month, the LSM 900 Live Cell will be exchanged for the LSM 910 with Lightfield 4D. There will be only slight differences in the confocal features, but with the added capabilities of Lightfield. If you are an LSM 900 Live Cell user, please transfer all your data to the shared drive by and watch for updates via email.

Image of the Month

We will be accepting an image of the month to be included in our newsletter.  It can be beautiful, the ugliest image you’ve ever taken, or funny. Internal Harvard users can submit their images here, and external users can email us at hcbi_imaging@fas.harvard.edu

Upcoming Events

Nikon Elements JOBS Seminar: October 28th

Are you a Nikon Spinning Disk user? Come learn from Nikon what workflows you can create with their graphical programming software, “JOBS.” JOBS allows you to create custom experiments, like taking a low-magnification image of the whole slide and then selecting an area to image in higher resolution or scanning multiwell plates.

Member Publications

Patel OH, Weisman AS, Hunter CP. A simple fixation for detection of endogenous fluorescent reporters in C. elegans that nearly eliminates intestinal autofluorescence. MicroPubl Biol. (2025)

Heller E et al. The membrane skeleton is constitutively remodeled in neurons by calcium signaling. Science 389, eadn6712(2025).

May EJ, & Gaudet R,  Surface delivery quantification reveals distinct trafficking efficiencies among clustered protocadherin isoforms, Proc. Natl. Acad. Sci. U.S.A. 122 (31) e2514178122, (2025).

Nelson AC et al., Molecular and neural control of social hierarchy by a forebrain-thalamocortical circuit. Cell S0092867425008104 (2025)

Senevirathne, G., Fernandopulle, S.C., Richard, D. et al. The evolution of hominin bipedalism in two steps. Nature (2025).

Super User of the Month

Alix Weisman (Hunter Lab) was imaging Halo-tagged SID-1 in C. elegans mutant backgrounds. Utilizing tile regions on the LSM980 and a custom JOBS script on the SoRa she was able to efficiently image samples day and night, allowing us to collect ~10-weeks of data in a 2-week span.