Nikon SoRa Spinning Disk Microscope

Overview:

The Nikon Super Resolution Spinning Disk Confocal (SoRa) is a fully-loaded live cell imaging platform with dual disks (SoRa 50 μm) for super resolution and standard confocal imaging, Hamamatsu Quest 2 qCMOS & Andor iXon 888 EMCCD and, full incubation. The system is capable of up to 120nm resolution with the SoRa disk and 10s of nm with SMLM.  DNA-paint and some dyes are possible for SMLM, but please speak to the imaging team prior to your experiment.

Specifications:

• Nikon Ti2E Inverted Microscope
• Yokogawa CSU-W1 dual-disk spinning disk with 25 μm & 50 μm pinholes
• Dual camera
• Motorized stage + piezo drive
• Perfect Focus System
• Full incubation with OKO Labs chamber

Objectives:

• Plan-Apochromat 4x/0.2 WD=20mm
• Plan-Apochromat 10x/0.45 WD=4mm
• Plan-Apochromat 25x/1.05 Silicon WD=0.55mm
• Apochromat Lambda S LWD 40x/1.15 Water WD=0.59mm
• Plan-Apochromat 60x/1.42 Oil WD=0.15mm
• Apochromat TIRF 60x/1.49 Oil WD=0.12

Lasers:

Laser Lines: 405,488,561,640

Filters:

Emission Filters:

Transmitting Dichroic:

• 405/488/568/647
• 405/488/568

Cameras:

Hamamatsu ORCA-Quest2 4096 x 2304 pixels, 4.6 µm pixel size

Andor iXon Ultra 888 EMCCD, 1024x1024pixels, 13um pixel