Abstract:
Hard tissue formation patterns and rates reveal details of animal physiology, life history, and environment, but are understudied in reptiles. Here, we use fluorescence labels delivered in vivo and laser confocal scanning microscopy to study tooth and bone formation in a managed group of green iguanas (Iguana iguana, Linne 1758) kept for 1.5 years under experimentally controlled conditions and undergoing several dietary switches. We constrain rates of tooth elongation, which we observe to be slow when enamel is initially deposited (c. 9 microm/day), but then increases exponentially in the dentin root, reaching c. 55 microm/day or more after crown completion. We further constrain the total timing of tooth formation to approximately 40-60 days, and observe highly variable timings of tooth resorption onset and replacement. Fluorescent labels clearly indicate cohorts of teeth recruited within Zahnreihen replacement waves, with faster sequential tooth recruitment and greater wave sizes posteriorly, where each wave initiates. Rates of hard tissue formation in long bones range from 0.4-3.4 microm/day, correlating with animal weight gain, and cortical bone recording the entire history of the experiment. We suggest additional labeling experiments to study hard tissue formation patterns in other reptiles, and propose strategies for chemical analyses of hard tissues in order to extract temporal information about past environments, behaviors and diets from reptilian fossils throughout the Phanerozoic.
Notes:
Green, Daniel R Winkler, Daniela E Leichliter, Jennifer Harms, Gregory S Hatt, Jean-Michel Clauss, Marcus Tutken, Thomas eng England 2023/07/21 Integr Comp Biol. 2023 Jul 20:icad089. doi: 10.1093/icb/icad089.
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