Abstract:
Embedded bioprinting enables the rapid design and fabrication of complex tissues that recapitulate in vivo microenvironments. However, few biological matrices enable good print fidelity, while simultaneously facilitate cell viability, proliferation, and migration. Here, a new microporogen-structured (microPOROS) matrix for embedded bioprinting is introduced, in which matrix rheology, printing behavior, and porosity are tailored by adding sacrificial microparticles composed of a gelatin-chitosan complex to a prepolymer collagen solution. To demonstrate its utility, a 3D tumor model is created via embedded printing of a murine melanoma cell ink within the microPOROS collagen matrix at 4 degrees C. The collagen matrix is subsequently crosslinked around the microparticles upon warming to 21 degrees C, followed by their melting and removal at 37 degrees C. This process results in a microPOROS matrix with a fibrillar collagen type-I network akin to that observed in vivo. Printed tumor cells remain viable and proliferate, while antigen-specific cytotoxic T cells incorporated in the matrix migrate to the tumor site, where they induce cell death. The integration of the microPOROS matrix with embedded bioprinting opens new avenues for creating complex tissue microenvironments in vitro that may find widespread use in drug discovery, disease modeling, and tissue engineering for therapeutic use.
Notes:
Reynolds, Daniel S de Lazaro, Irene Blache, Manon L Liu, Yutong Jeffreys, Nicholas C Doolittle, Ramsey M Grandidier, Estee Olszewski, Jason Dacus, Mason T Mooney, David J Lewis, Jennifer A eng U01CA21436901/CA/NCI NIH HHS/ Germany 2023/05/10 Adv Mater. 2023 May 10:e2210748. doi: 10.1002/adma.202210748.
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