Zeiss LSM 980 Confocal Microscope with Airyscan 2

Overview:

The LSM 980 confocal microscope offers 34-channels of supreme sensitivity, owing to its GaAsP and Airyscan2 detectors. This extraordinary point scanner can simultaneously image and spectrally separate up to 10 different dyes and create high-resolution 3D images over large tiled areas. The unparalleled sensitivity of this system also makes it the ideal live-cell imaging point scanner, allowing for high-speed confocal acquisition with brilliant signal-to-noise ratios. Along with deconvolution, Airyscan2 provides a 1.7x improvement over the theoretical diffraction limit. This system also utilizes the Airyscan2 detector for Zeiss Dynamic Profiler to measure molecular concentration, asymmetric diffusion, and flow dynamics of fluorescent proteins in your living samples.

Specifications:

  • Zeiss Axio Observer 7 Inverted Microscope
  • Motorized Stage
  • Definite Focus 3
  • Full Incubation
  • Airyscan2 with 8Y Mode

Objectives (Updated August 2025):

  • 10x air ( C-Apochromat 10x/0.45 WD=1.8mm)
  • 20x air (Plan Apochromat 20x/0.8 WD=0.55mm)
  • 40x water ((LD)C-Apochromat 40x/1.1  WD=0.62mm)
  • 40x oil (Plan-Apochroma 40x/1.3 Oil DIC  WD=0.2mm)
  • 63x oil (Plan-Apochromat 63x/1.40 Oil DIC WD=0.19mm)

Lasers:

Laser lines: 405, 445, 488, 514, 561, 594, 640, 730
Commonly imaged dyes: all visible spectrum and near IR dyes (405-750nm)

Detectors: 

  • 2 MA-PMT
  • 32 channel GaAsP array
  • NIR "BIG" detector
  • Airyscan 2
  • T-PMT for transmitted light