Zeiss LSM 980 NLO Multi-Photon Microscope

Overview:

Multi-photon illumination has always appealed to biologists for imaging deep into living tissue. But did you ever imagine probing more than 5mm into your sample? Using the 980 NLO with our specialized cleared tissue objective, you can visualize deep into clarified tissue with incredible resolution. Even without clarifying your samples, this microscope excels at penetrating deep into live or fixed specimens, and creating beautiful 3D datasets thanks to a tunable infra-red laser that can be used for multi-photon (2P, 3P) and second harmonic generation (SHG) imaging.

Specifications:

• Zeiss Examiner Upright Fixed Stage Microscope
• Motorized Stage and Z-drive

• Adjustable height stage for small and large samples

   

Objectives

• 10x air (Plan Apochromat 10x/0.45 WD=2.0 mm)
• 25x multi-immersion (LD LCI Plan Apochromat 25x/0.8 WD=0.57 mm)
• 10x water dipping (W Plan Apochromat 10x/0.5 WD=3.7 mm )
• 20x water dipping (W Plan Apochromat 20x/1.0 WD=2.4 mm)
• 20x clearing objective (Clr Plan-Neofluar 20x/1.0 WD=6.4 mm)

Lasers:

Laser lines (confocal): 405, 440, 458, 488, 514, 561, 633
Laser lines (2-photon): Spectra-Physics Insight DS+ tunable from 680-1300nm
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)
 

Detectors:

Confocal detection: 2 MA-PMT + 4 channel GaAsP detector
2-photon detection: 6 channel spectral detector or 4 channel (Blue, Green, Red, Far-Red) non-descanned detection